Extraction and recombination studies of the interaction of retinol with human plasma retinol-binding protein
نویسندگان
چکیده
منابع مشابه
The interaction of human plasma retinol-binding protein and prealbumin.
The interaction of human plasma retinol-binding protein with plasma prealbumin was studied by the techniques of velocity ultracentrifugation and polarization of retinol fluorescence. In the first method the unbound fraction of retinol-binding protein, which sediments more slowly than its complexes with prealbumin, was measured by its absorption. A stoichiometry of retinol-binding protein to pre...
متن کاملRadioimmunoassay of human plasma retinol-binding protein.
A radioimmunoassay for human plasma retinol-binding protein (RBP) has been developed utilizing a double antibody precipitation technique. RBP was purified 1500- to 2000-fold by procedures described previously. A specific anti-human RBP antiserum was prepared in rabbits by three once-weekly injections of purified RBP emulsified with Freund's adjuvant. RBP was iodinated with (131)I and the RBP-(1...
متن کاملThe interaction of thyroxine with human plasma prealbumin and with the prealbumin-retinol-binding protein complex.
Prealbumin was isolated from human plasma by chromatography on columns of diethylaminoethyl Sephadex and Sephadex G-200, followed by preparative polyacrylamide gel electrophoresis. The prealbumin was homogeneous in the analytical ultracentrifuge with an s=z~,~ of 3.7 S and with a molecular weight of about 50,000. Prealbumin formed a protein-protein complex with plasma retinol-binding protein in...
متن کاملLoss of retinol-binding properties for plasma retinol-binding protein in normal human epidermis.
Terminal differentiation of the keratinocytes (cornification) has been linked to a restricted supply of retinol. Retinol is distributed to target cells by the retinol-binding protein (RBP), which circulates in the plasma in complex with transthyretin (TTR). In this study we have addressed the question of retinol delivery to the epidermis via RBP. Retinol radiobinding assays, affinity chromatogr...
متن کاملInteraction of the retinol/cellular retinol-binding protein complex with isolated nuclei and nuclear components
Retinol (vitamin A alcohol) is involved in the proper differentiation of epithelia. The mechanism of this involvement is unknown. We have previously reported that purified cellular retinol-binding (CRBP) will mediate specific binding of retinol to nuclei isolated from rat liver. We now report that pure CRBP delivers retinol to the specific nuclear binding sites without itself remaining bound. T...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1972
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)39396-2